Experimental and model investigation of the time-dependent 2-dimensional distribution of binding in a herringbone microchannel.
نویسندگان
چکیده
A microfluidic device known to mix bulk solutions, the herringbone microchannel, was incorporated into a surface-binding assay to determine if the recirculation of solution altered the binding of a model protein (streptavidin) to the surface. Streptavidin solutions were pumped over surfaces functionalized with its ligand, biotin, and the binding of streptavidin to those surfaces was monitored using surface plasmon resonance imaging. Surface binding was compared between a straight microchannel and herringbone microchannels in which the chevrons were oriented with and against the flow direction. A 3-dimensional finite-element model of the surface binding reaction was developed for each of the geometries and showed strong qualitative agreement with the experimental results. Experimental and model results indicated that the forward and reverse herringbone microchannels substantially altered the distribution of protein binding (2-dimensional binding profile) as a function of time when compared to a straight microchannel. Over short distances (less than 1.5 mm) down the length of the microchannel, the model predicted no additional protein binding in the herringbone microchannel compared to the straight microchannel, consistent with previous findings in the literature.
منابع مشابه
Supplementary information: Experimental and model investigation of the time-dependent 2-dimensional distribution of binding in a herringbone microchannel
The model results for the velocity field of the herringbone microchannel (Figure 1) indicate that the velocity of the fluid within the herringbone was significantly lower than the 10 velocity in the base microchannel. The velocity profile in the base microchannel closely resembles the parabolic velocity profile in the straight microchannel. However, unlike the uni-directional parabolic velocity...
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عنوان ژورنال:
- Lab on a chip
دوره 8 4 شماره
صفحات -
تاریخ انتشار 2008